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KMID : 0361620030380060624
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Journal of the Korean Orthopaedic Association
2003 Volume.38 No. 6 p.624 ~ p.630
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Effect of Polyethylene Particles on Osteoblasts in Pathogenesis of Osteolysis
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Abstract
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Purpose: To determirie the role of osteoblast in the pathogenesis of osteoysis, we evaluated cell prolrferaiion and differentiation of cultured osteoblasts by adding retrieved polyethylene particles.
Materials and Methods: We cultured the osteoblasts primarily isolated from rat neonate calvariums and then added polyethyler~ particles - retrjeved from osteoysis tissue. We evaluated cell proliferation, alkaline phospf>atase activiy, the expression of Type J procoNagen mRNA, the expression of ostegcalcjn mRNA, gone nodule formation, and the expression bf osteoprotegerin (OPG) mRNA-and receptor activator of NF-kappa B ligand (RANKL) mRNA according to culture periods.
Results: Particle groups showed statistically significant decrease in cell proliferation after the 7th day. The expression of type I procollagen mRNA increased on the 14th day. The bone nodules appeared at the 7th day and increased with time. There was no appearance of bone nocfule in the control group. The expression of OPG mRNA in particle groups was not changed. The expression of RANKL mRNA was significantly increased in high concentration particle group (4 mglmL) on the 14th day.
Conclusion: Polyethylene particles ceased the prol´rferation of osteoblasts and produced early extracellular matrix maturation and mineralization process in vitro and increased the expression of RANKU promoting osteoclast activities.
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KEYWORD
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